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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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SLC6A3
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

SLC6A3

Gene description

Solute carrier family 6 (neurotransmitter transporter), member 3

Protein class

Disease related genes
FDA approved drug targets
Predicted membrane proteins
Transporters

Predicted localization

Membrane

Number of transcripts

1
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Tissue enriched (renal cancer)
HPA (cell line):Not detected
HPA (normal tissue):Not detected

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Estimation of protein expression could not be performed. View primary data.

ANTIBODY IHC RELIABILITY

Data reliability
description

RNA-based expert annotation gave inconclusive results. Pending retesting on additional tissue. No internal RNA expression data available for correlation.

Reliability score

Uncertain based on 2 antibodies.
HPA013602 , CAB016249
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable).
Renal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Tissue enriched (renal cancer)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Endometrial cancer

STAINING SUMMARY

HPA013602
CAB016249

Cancer

Cancer staining

Breast cancer
Carcinoid
Cervical cancer
Colorectal cancer
Endometrial cancer
Glioma
Head and neck cancer
Liver cancer
Lung cancer
Lymphoma
 

Cancer

Cancer staining

Melanoma
Ovarian cancer
Pancreatic cancer
Prostate cancer
Renal cancer
Skin cancer
Stomach cancer
Testis cancer
Thyroid cancer
Urothelial cancer

Staining summary

Among tumors, hepatocellular cancers, some adenocarcinomas and non-Hodgkins lymphomas and a small fraction of squamous cell cancers displayed strong cytoplasmic immunoreactivity. Other malignancies were generally moderately stained.

Cancer

Cancer staining

Breast cancer
Carcinoid
Cervical cancer
Colorectal cancer
Endometrial cancer
Glioma
Head and neck cancer
Liver cancer
Lung cancer
Lymphoma
 

Cancer

Cancer staining

Melanoma
Ovarian cancer
Pancreatic cancer
Prostate cancer
Renal cancer
Skin cancer
Stomach cancer
Testis cancer
Thyroid cancer
Urothelial cancer

Staining summary

Tumor tissues exhibited weak to moderate cytoplasmic positivity. Renal cell carcinomas showed strong nuclear and cytoplasmic positivity. Some malignant carcinoids also displayed strong cytoplasmic staining. Squamous cell carcinomas, stomach and liver cancers were mainly weakly stained or negative.

GENE INFORMATION

Gene name

SLC6A3 (HGNC Symbol)

Synonyms

DAT, DAT1

Description

Solute carrier family 6 (neurotransmitter transporter), member 3 (HGNC Symbol)

Entrez gene summary

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Chromosome

5

Cytoband

p15.33

Chromosome location (bp)

1392790 - 1445430

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000142319 (version 83.38)

Entrez gene

6531

UniProt

Q01959 (UniProt - Evidence at protein level)

neXtProt

NX_Q01959

Antibodypedia

SLC6A3 antibodies

PROTEIN BROWSER

SLC6A3-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

SLC6A3-001
ENSP00000270349
ENST00000270349
Q01959 [Direct mapping]
Sodium-dependent dopamine transporter
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Transporters
   Electrochemical Potential-driven transporters
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   >9TM proteins predicted by MDM
Disease related genes
FDA approved drug targets
   Small molecule drugs
Show all
GO:0002020 [protease binding]
GO:0005102 [receptor binding]
GO:0005328 [neurotransmitter:sodium symporter activity]
GO:0005329 [dopamine transmembrane transporter activity]
GO:0005330 [dopamine:sodium symporter activity]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006836 [neurotransmitter transport]
GO:0007268 [synaptic transmission]
GO:0007568 [aging]
GO:0007595 [lactation]
GO:0007608 [sensory perception of smell]
GO:0007626 [locomotory behavior]
GO:0008144 [drug binding]
GO:0008504 [monoamine transmembrane transporter activity]
GO:0009986 [cell surface]
GO:0010039 [response to iron ion]
GO:0014070 [response to organic cyclic compound]
GO:0015844 [monoamine transport]
GO:0015872 [dopamine transport]
GO:0016021 [integral component of membrane]
GO:0016600 [flotillin complex]
GO:0021984 [adenohypophysis development]
GO:0030424 [axon]
GO:0032403 [protein complex binding]
GO:0035094 [response to nicotine]
GO:0035240 [dopamine binding]
GO:0040018 [positive regulation of multicellular organism growth]
GO:0042053 [regulation of dopamine metabolic process]
GO:0042136 [neurotransmitter biosynthetic process]
GO:0042220 [response to cocaine]
GO:0042416 [dopamine biosynthetic process]
GO:0042420 [dopamine catabolic process]
GO:0042493 [response to drug]
GO:0043005 [neuron projection]
GO:0043025 [neuronal cell body]
GO:0045471 [response to ethanol]
GO:0047485 [protein N-terminus binding]
GO:0051583 [dopamine uptake involved in synaptic transmission]
GO:0051591 [response to cAMP]
GO:0051721 [protein phosphatase 2A binding]
GO:0055085 [transmembrane transport]
GO:0060134 [prepulse inhibition]
GO:0072488 [ammonium transmembrane transport]
Show all
620 aa
68.5 kDa
No >9

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