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KLK3

ANTIBODY INFORMATION
 
 

Antibody HPA000764

  

Antibody CAB000070

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA000764   A0562  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 KLK2 - ENSG00000167751 [80%]
    

Released in version

 1   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA000764, RRID:AB_1079700    

VALIDATION SUMMARY
 
 

Antibody HPA000764

  

Antibody CAB000070

  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000764

  

Antibody CAB000070

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 2 cell types		   RNA: detected in 21 tissues
Protein: detected in 2 cell types		  

Retrieval

 None   None  

Antibody dilution

 1:500   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000764

  

Antibody CAB000070

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 28.7, 28.6, 26.3, 25.1, 23.7, 19.1   28.7, 28.6, 26.3, 25.1, 24.3, 23.7, 21.3, 19.2, 19.1  

Antibody dilution

 1:250   1:5000  

PROTEIN ARRAY
 
 

Antibody HPA000764

  

Antibody CAB000070

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA000764

  

Antibody CAB000070

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 143    

Antigen sequence

 
SHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLP
TQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKV
TKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCA
   

Matching transcripts

 KLK3-003 - ENSP00000314151 [100%]
KLK3-014 - ENSP00000471827 [95%]
KLK3-015 - ENSP00000471155 [86%]
KLK3-007 - ENSP00000353829 [83%]
KLK3-010 - ENSP00000472907 [83%]
KLK3-013 - ENSP00000470523 [82%]
   

Other gene match

 KLK2 - ENSG00000167751 [80%]
    
ANTIGEN VIEW
KLK3-003
KLK3-007
KLK3-010
KLK3-013
KLK3-014
KLK3-015
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.