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FAM71E1

ANTIBODY INFORMATION
 
 

Antibody HPA048111

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048111  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA048111

  
Immunocytochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048111

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center.  

Antibody dilution

 1:22  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA048111

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 27.6, 25.8  

Antibody dilution

 1:110  

PROTEIN ARRAY
 
 

Antibody HPA048111

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:900  

ANTIGEN INFORMATION
 
 

Antibody HPA048111

  

Antigen

 Recombinant protein fragment  

Length (aa)

 83  

Antigen sequence

 
PGGMAASPTGRPRRLQRYLQSGEFDQFRDFPIFESNFVQFCPDIYPAPTS
DLWPQVTRLGEVANEVTMGVAASSPALELPDLL
 

Matching transcripts

 FAM71E1-001 - ENSP00000472421 [100%]
FAM71E1-002 - ENSP00000471272 [81%]
 

Other gene match

  
ANTIGEN VIEW
FAM71E1-001
FAM71E1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.