Cell atlas - NOSIP - The Human Protein Atlas

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NOSIP

TISSUE

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CELL

PATHOLOGY

CELL ATLAS RNA EXPRESSION HUMAN CELLS siRNA

GENE/PROTEIN

Antibody validation

Dictionary
	Nucleus

Human cell
	Nucleoplasm

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GENERAL INFORMATION
Gene name	NOSIP
Gene description	Nitric oxide synthase interacting protein
Protein class	Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleus.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleus (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA043464 , HPA062132
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleus.

Main location

Nucleus (validated)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	HPA043464	A-431	Nucleoplasm
	HPA043464	U-2 OS	Nucleus
	HPA043464	U-251 MG	Nucleoplasm
	HPA062132	HEK 293	Nucleus
	HPA062132	PC-3	Nucleoplasm
	HPA062132	U-2 OS	Nucleus

siRNA VALIDATION

Antibody

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

53%

61%

p-value

< 0.01

< 0.01

Antibody

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 2

RFI

37%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA043464	siRNA 1	U-2 OS
		HPA043464	siRNA 2	U-2 OS
		HPA043464	scrambled	U-2 OS
		HPA062132	siRNA 2	U-2 OS
		HPA062132	scrambled	U-2 OS

GENE INFORMATION
Gene name	NOSIP (HGNC Symbol)
Synonyms	CGI-25
Description	Nitric oxide synthase interacting protein (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]
Chromosome	19
Cytoband	q13.33
Chromosome location (bp)	49555711 - 49590262
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000142546 (version 83.38)
Entrez gene	51070
UniProt	Q9Y314 (UniProt - Evidence at protein level)
neXtProt	NX_Q9Y314
Antibodypedia	NOSIP antibodies

PROTEIN BROWSER

NOSIP-001	NOSIP-004	NOSIP-005	NOSIP-008	NOSIP-010

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
NOSIP-001 ENSP00000470034 ENST00000596358	Q9Y314 [Direct mapping] Nitric oxide synthase-interacting protein Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000139 [Golgi membrane] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0043086 [negative regulation of catalytic activity] GO:0044281 [small molecule metabolic process] GO:0044822 [poly(A) RNA binding] GO:0046209 [nitric oxide metabolic process] GO:0050999 [regulation of nitric-oxide synthase activity] GO:0051001 [negative regulation of nitric-oxide synthase activity] Show all	301 aa 33.2 kDa	No	0
NOSIP-004 ENSP00000473109 ENST00000599537	M0R3B2 [Direct mapping] Nitric oxide synthase-interacting protein Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0050999 [regulation of nitric-oxide synthase activity] Show all	242 aa 26.9 kDa	No	0
NOSIP-005 ENSP00000468974 ENST00000598544	M0QX85 [Direct mapping] Nitric oxide synthase-interacting protein Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004842 [ubiquitin-protein transferase activity] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0016567 [protein ubiquitination] GO:0050999 [regulation of nitric-oxide synthase activity] Show all	96 aa 11.1 kDa	No	0
NOSIP-008 ENSP00000343497 ENST00000339093	A0A075B6F9 [Direct mapping] Nitric oxide synthase-interacting protein Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0050999 [regulation of nitric-oxide synthase activity] Show all	304 aa 33.4 kDa	No	0
NOSIP-010 ENSP00000471929 ENST00000598296	M0R1K2 [Direct mapping] Nitric oxide synthase-interacting protein Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0050999 [regulation of nitric-oxide synthase activity] Show all	176 aa 19.7 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.