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BARHL2

ANTIBODY INFORMATION
 
 

Antibody HPA028176

  

Antibody HPA031326

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028176   HPA031326  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA028176, RRID:AB_10601119   Atlas Antibodies Cat#HPA031326, RRID:AB_2673828  

VALIDATION SUMMARY
 
 

Antibody HPA028176

  

Antibody HPA031326

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028176

  

Antibody HPA031326

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.   Immunohistochemical staining of human rectum shows moderate nuclear and luminal membranous positivity in glandular cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 70 cell types		   RNA: detected in 1 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:600  

Literature conformity

 Not consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028176

  

Antibody HPA031326

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 42   42  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028176

  

Antibody HPA031326

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028176

  

Antibody HPA031326

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   64  

Antigen sequence

 
ILGDSKPLAACAPYSTSVSSPHHTPKQESNAVHESFRPKLEQEDSKTKLD
KREDSQSDIKCHGTKEEGDREITSSR
  
YHPSLLGSMDSTTAAAAAAAMYSSMYRTPPAPHPQLQRPLVPRVLIHGLG
PGGQPALNPLSSPI
 

Matching transcripts

 BARHL2-001 - ENSP00000359474 [100%]
  BARHL2-001 - ENSP00000359474 [100%]
 

Other gene match

    
ANTIGEN VIEW
BARHL2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.