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MTF2

ANTIBODY INFORMATION
 
 

Antibody HPA069047

  

Antibody HPA069066

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA069047   HPA069066  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA069047, RRID:AB_2686073   Atlas Antibodies Cat#HPA069066, RRID:AB_2686078  

VALIDATION SUMMARY
 
 

Antibody HPA069047

  

Antibody HPA069066

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA069047

  

Antibody HPA069066

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA069066.		  
Validated

Antibody staining overlaps with antibody HPA069047.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.  

Antibody dilution

 1:161   1:96  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA069047

  

Antibody HPA069066

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.1, 60.6, 55.7   67.1, 60.6, 55.7  

Antibody dilution

 1:530   1:320  

PROTEIN ARRAY
 
 

Antibody HPA069047

  

Antibody HPA069066

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6400   1:3850  

ANTIGEN INFORMATION
 
 

Antibody HPA069047

  

Antibody HPA069066

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   68  

Antigen sequence

 
GRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSW
PAAIPHLRRRRGRLPRRALQTQNSEIVKDDE
  
AHLCLYNLSVIHKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEH
VLEALNDYKTMFMSGKEI
 

Matching transcripts

 MTF2-001 - ENSP00000359321 [100%]
MTF2-003 - ENSP00000359326 [100%]
MTF2-201 - ENSP00000443295 [100%]
MTF2-202 - ENSP00000444962 [100%]
  MTF2-001 - ENSP00000359321 [100%]
MTF2-201 - ENSP00000443295 [100%]
MTF2-202 - ENSP00000444962 [100%]
MTF2-003 - ENSP00000359326 [91%]
 

Other gene match

    
ANTIGEN VIEW
MTF2-001
MTF2-003
MTF2-201
MTF2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.