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GPA33

ANTIBODY INFORMATION
 
 

Antibody HPA018858

  

Antibody CAB025943

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA018858   MAB3080  

Host species

 Rabbit   Rat  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   5  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA018858, RRID:AB_1849909    

VALIDATION SUMMARY
 
 

Antibody HPA018858

  

Antibody CAB025943

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018858

  

Antibody CAB025943

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows distinct cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human colon shows strong membranous positivity in glandular cells.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 5 cell types		   RNA: detected in 11 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018858

  

Antibody CAB025943

  

Standard validation

 Uncertain

Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417048)		    

Target mass (kDa)

 35.6   35.6, 7.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018858

  

Antibody CAB025943

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018858

  

Antibody CAB025943

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 114    

Antigen sequence

 
TYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQ
SKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTS
SNEEGTQFCNITVA
  

											 
																				

																		

										
											
Matching transcripts

												
																					GPA33-001 - ENSP00000356842 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GPA33-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA018858

												
										 
																				
											
											
											
Antibody CAB025943

												
										 
																		

																	

										
											
1.

												
																						Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas

	Wu CC et al

	Mol Cell Proteomics 2010;9(6):1100-17

			
											 
																						
											 
																				

																		

										
											
2.

												
																						A recellularized human colon model identifies cancer driver genes

	Chen HJ et al

	Nat Biotechnol 2016;34(8):845-51

			Application: ICC-IF				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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