ARNT - Antibodies - The Human Protein Atlas

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ARNT

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA001759

Antibody CAB004318

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA001759

sc-17811

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA001759, RRID:AB_1078213

Santa Cruz Biotechnology Cat#sc-17811, RRID:AB_626698

VALIDATION SUMMARY

Antibody HPA001759

Antibody CAB004318

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA001759

Antibody CAB004318

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nuclear bodies.

Antibody dilution

1:19

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA001759

Antibody CAB004318

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 62 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001759

Antibody CAB004318

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

86.6, 86.4, 85.3

86.6, 86.4, 85.3, 84.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001759

Antibody CAB004318

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

ANTIGEN INFORMATION

Antibody HPA001759

Antibody CAB004318

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

121

Antigen sequence

RFSCLRPRVAGTTEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRP
GLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHS
EIERRRRNKMTAYITELSDMV

Matching transcripts

ARNT-001 - ENSP00000351407 [90%]
ARNT-010 - ENSP00000346372 [90%]
ARNT-006 - ENSP00000423851 [88%]

Other gene match

ANTIGEN VIEW

ARNT-001	ARNT-006	ARNT-010

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.