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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Antibody dilution
1:105
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Antibody dilution
1:105
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA036241
Antibody HPA036242
Antibody HPA066090
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human esophagus shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 39 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036241
Antibody HPA036242
Antibody HPA066090
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408651)
Target mass (kDa)
53.7, 41.9
53.7, 41.9
53.7
Antibody dilution
1:250
1:250
1:350
PROTEIN ARRAY
Antibody HPA036241
Antibody HPA036242
Antibody HPA066090
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.