Expression of PAM in cancer - Summary - The Human Protein Atlas

GENERAL INFORMATION
Gene name	PAM
Gene description	Peptidylglycine alpha-amidating monooxygenase
Protein class	Enzymes Plasma proteins Predicted intracellular proteins Predicted membrane proteins Predicted secreted proteins
Predicted localization	Intracellular,Membrane,Secreted
Number of transcripts	13
	SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Mixed
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in neuroendocrine cells, islets of Langerhans and heart muscle.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Secreted protein, tissue location of RNA and protein might differ and correlation is complex.
Reliability score	Supported based on 2 antibodies. HPA042260 , CAB026119
	SHOW MORE

PROGNOSTIC SUMMARY
Prognostic marker in endometrial cancer (favourable) and liver cancer (unfavourable).
Endometrial cancer p<0.001 Liver cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA042260	CAB026119

GENE INFORMATION
Gene name	PAM (HGNC Symbol)
Synonyms	PAL, PHM
Description	Peptidylglycine alpha-amidating monooxygenase (HGNC Symbol)
Entrez gene summary	This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq, Jul 2008]
Chromosome	5
Cytoband	q21.1
Chromosome location (bp)	102753981 - 103031105
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000145730 (version 83.38)
Entrez gene	5066
UniProt	P19021 (UniProt - Evidence at protein level)
neXtProt	NX_P19021
Antibodypedia	PAM antibodies

PROTEIN BROWSER

PAM-001	PAM-002	PAM-003	PAM-004	PAM-005	PAM-008	PAM-010	PAM-012	PAM-016	PAM-017	PAM-018	PAM-019	PAM-029

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
PAM-001 ENSP00000314638 ENST00000348126	P19021 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating monooxygenase Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Show all	Enzymes ENZYME proteins Oxidoreductases Lyases Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001519 [peptide amidation] GO:0003824 [catalytic activity] GO:0004497 [monooxygenase activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0004598 [peptidylamidoglycolate lyase activity] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0008270 [zinc ion binding] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0031418 [L-ascorbic acid binding] GO:0055114 [oxidation-reduction process] GO:0070062 [extracellular exosome] Show all	866 aa 96.3 kDa	Yes	1
PAM-002 ENSP00000403461 ENST00000455264	P19021 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating monooxygenase Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Show all	Enzymes ENZYME proteins Oxidoreductases Lyases MEMSAT3 predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001519 [peptide amidation] GO:0003824 [catalytic activity] GO:0004497 [monooxygenase activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0004598 [peptidylamidoglycolate lyase activity] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0008270 [zinc ion binding] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0031418 [L-ascorbic acid binding] GO:0055114 [oxidation-reduction process] GO:0070062 [extracellular exosome] Show all	905 aa 100.8 kDa	Yes	0
PAM-003 ENSP00000282992 ENST00000346918	P19021 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating monooxygenase Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Show all	Enzymes ENZYME proteins Oxidoreductases Lyases MEMSAT3 predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001519 [peptide amidation] GO:0003824 [catalytic activity] GO:0004497 [monooxygenase activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0004598 [peptidylamidoglycolate lyase activity] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0008270 [zinc ion binding] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0031418 [L-ascorbic acid binding] GO:0055114 [oxidation-reduction process] GO:0070062 [extracellular exosome] Show all	887 aa 98.8 kDa	Yes	0
PAM-004 ENSP00000396493 ENST00000438793	P19021 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating monooxygenase Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Show all	Enzymes ENZYME proteins Oxidoreductases Lyases Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001519 [peptide amidation] GO:0003824 [catalytic activity] GO:0004497 [monooxygenase activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0004598 [peptidylamidoglycolate lyase activity] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0008270 [zinc ion binding] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0031418 [L-ascorbic acid binding] GO:0055114 [oxidation-reduction process] GO:0070062 [extracellular exosome] Show all	973 aa 108.3 kDa	Yes	1
PAM-005 ENSP00000369125 ENST00000379799	H7BYD9 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003824 [catalytic activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0005507 [copper ion binding] GO:0005515 [protein binding] GO:0006518 [peptide metabolic process] GO:0016020 [membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0055114 [oxidation-reduction process] Show all	679 aa 75.6 kDa	No	0
PAM-008 ENSP00000424203 ENST00000504691	H0Y9I4 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0016021 [integral component of membrane] Show all	250 aa 27.6 kDa	No	1
PAM-010 ENSP00000420851 ENST00000512073	D6RDU2 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		25 aa 2.7 kDa	Yes	0
PAM-012 ENSP00000421569 ENST00000505654	D6RF09 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		29 aa 3.1 kDa	Yes	0
PAM-016 ENSP00000426448 ENST00000511839	D6RG20 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		68 aa 7.5 kDa	Yes	0
PAM-017 ENSP00000421823 ENST00000511477	D6RAQ2 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		42 aa 4.7 kDa	Yes	0
PAM-018 ENSP00000423611 ENST00000506006	D6R961 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	MEMSAT-SVM predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		28 aa 3 kDa	Yes	0
PAM-019 ENSP00000423763 ENST00000509832	D6RCD5 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all		53 aa 5.9 kDa	Yes	0
PAM-029 ENSP00000306100 ENST00000304400	P19021 [Direct mapping] Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating monooxygenase Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Show all	Enzymes ENZYME proteins Oxidoreductases Lyases Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001519 [peptide amidation] GO:0001666 [response to hypoxia] GO:0001676 [long-chain fatty acid metabolic process] GO:0003824 [catalytic activity] GO:0004497 [monooxygenase activity] GO:0004504 [peptidylglycine monooxygenase activity] GO:0004598 [peptidylamidoglycolate lyase activity] GO:0005507 [copper ion binding] GO:0005509 [calcium ion binding] GO:0005515 [protein binding] GO:0005615 [extracellular space] GO:0005802 [trans-Golgi network] GO:0005886 [plasma membrane] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006518 [peptide metabolic process] GO:0007417 [central nervous system development] GO:0007507 [heart development] GO:0007595 [lactation] GO:0008270 [zinc ion binding] GO:0009268 [response to pH] GO:0009404 [toxin metabolic process] GO:0009986 [cell surface] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016715 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen] GO:0018032 [protein amidation] GO:0019538 [protein metabolic process] GO:0019901 [protein kinase binding] GO:0022602 [ovulation cycle process] GO:0030141 [secretory granule] GO:0030667 [secretory granule membrane] GO:0031418 [L-ascorbic acid binding] GO:0032355 [response to estradiol] GO:0032956 [regulation of actin cytoskeleton organization] GO:0042476 [odontogenesis] GO:0042493 [response to drug] GO:0043005 [neuron projection] GO:0043025 [neuronal cell body] GO:0043204 [perikaryon] GO:0046688 [response to copper ion] GO:0048471 [perinuclear region of cytoplasm] GO:0050708 [regulation of protein secretion] GO:0051260 [protein homooligomerization] GO:0051384 [response to glucocorticoid] GO:0055114 [oxidation-reduction process] GO:0060135 [maternal process involved in female pregnancy] GO:0060173 [limb development] GO:0070062 [extracellular exosome] Show all	974 aa 108.4 kDa	Yes	1