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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
Expression
RNA: detected in 32 tissues Protein: detected in 16 cell types
RNA: detected in 32 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:60
Literature conformity
No avaliable gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029145
Antibody HPA049692
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406469)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
74.9
74.9
Antibody dilution
1:250
1:240
PROTEIN ARRAY
Antibody HPA029145
Antibody HPA049692
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.