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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:100
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA029792
Antibody HPA029794
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Expression
RNA: detected in 18 tissues Protein: detected in 51 cell types
RNA: detected in 18 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:325
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029792
Antibody HPA029794
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408676)
Target mass (kDa)
43.4, 27.2
43.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA029792
Antibody HPA029794
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.