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AKAP4

ANTIBODY INFORMATION
 
 

Antibody HPA005949

  

Antibody HPA020046

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005949   HPA020046  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   5  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA005949, RRID:AB_1078120   Atlas Antibodies Cat#HPA020046, RRID:AB_1844693  

VALIDATION SUMMARY
 
 

Antibody HPA005949

  

Antibody HPA020046

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005949

  

Antibody HPA020046

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows moderate positivity in spermatids.   Immunohistochemical staining of human testis shows distinct positivity in developing sperm cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 9 cell types		   RNA: detected in 1 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1200   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005949

  

Antibody HPA020046

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 94.5, 93.4   94.5, 93.4, 28.3, 23.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA005949

  

Antibody HPA020046

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA005949

  

Antibody HPA020046

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   139  

Antigen sequence

 
SALKLIQYHLTQQTKGKDTCEEDCPGSTMGYMAQSTQYEKCGGGQSAKAL
SVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIVLMLIQKLLNENPFKCE
DPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQ
  
EIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQH
ALSPSTSTCKHKVGDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRL
EMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDGECSIDD
 

Matching transcripts

 AKAP4-001 - ENSP00000351327 [100%]
AKAP4-002 - ENSP00000365232 [100%]
  AKAP4-001 - ENSP00000351327 [100%]
AKAP4-002 - ENSP00000365232 [100%]
AKAP4-004 - ENSP00000412279 [100%]
AKAP4-003 - ENSP00000402403 [81%]
 

Other gene match

    
ANTIGEN VIEW
AKAP4-001
AKAP4-002
AKAP4-003
AKAP4-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA005949

  

Antibody HPA020046

  

1.

 The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Djureinovic D et al
Mol Hum Reprod 2014;20(6):476-88
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.