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Antibody staining overlaps with antibody HPA054559.
Validated
Antibody staining overlaps with antibody HPA054094.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Antibody dilution
1:141
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054094
Antibody HPA054559
Antibody CAB022069
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA054559 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA054094 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells, glial cells and endothelial cells, neuropil was negative.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity and additional strong cytoplasmic staining.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1400
1:75
1:1000
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054094
Antibody HPA054559
Antibody CAB022069
Formal validation: Genetic
Validated
Downregulation visible in both siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
