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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

OGT

Gene description

O-linked N-acetylglucosamine (GlcNAc) transferase

Protein class

Disease related genes
Enzymes
Potential drug targets
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and nuclear expression in all tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Pending external verification.

Reliability score

Approved based on 4 antibodies.
HPA030751 , HPA030752 , HPA030754 , CAB034099
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in urothelial cancer (favourable), renal cancer (unfavourable), head and neck cancer (favourable) and endometrial cancer (unfavourable).
Urothelial cancer p<0.001
Renal cancer p<0.001
Head and neck cancer p<0.001
Endometrial cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA030751
HPA030752
HPA030754
CAB034099

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Weak to moderate nuclear and cytoplasmic positivity was observed in most cancer tissues. Several cases of prostate and renal cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Hepatocellular carcinomas displayed moderate cytoplasmic immunoreactivity. Remaining cancer tissues were generally negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several hepatocellular carcinomas, renal cell carcinomas along with a few cases of gliomas, lymphomas and thyroid cancers showed moderate cytoplasmic positivity. Remaining cancer tissues were in general weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues showed weak to moderate nuclear immunoreactivity. A majority of renal, gastric, liver, lung and endometrial cancers were negative.

GENE INFORMATION

Gene name

OGT (HGNC Symbol)

Synonyms

FLJ23071, HRNT1, MGC22921, O-GLCNAC

Description

O-linked N-acetylglucosamine (GlcNAc) transferase (HGNC Symbol)

Entrez gene summary

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Chromosome

X

Cytoband

q13.1

Chromosome location (bp)

71533083 - 71575897

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000147162 (version 83.38)

Entrez gene

8473

UniProt

O15294 (UniProt - Evidence at protein level)

neXtProt

NX_O15294

Antibodypedia

OGT antibodies

PROTEIN BROWSER

OGT-001
OGT-002
OGT-011

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

OGT-001
ENSP00000362824
ENST00000373719
O15294 [Direct mapping]
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000123 [histone acetyltransferase complex]
GO:0000791 [euchromatin]
GO:0001933 [negative regulation of protein phosphorylation]
GO:0001934 [positive regulation of protein phosphorylation]
GO:0005515 [protein binding]
GO:0005547 [phosphatidylinositol-3,4,5-trisphosphate binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005815 [microtubule organizing center]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006041 [glucosamine metabolic process]
GO:0006110 [regulation of glycolytic process]
GO:0006325 [chromatin organization]
GO:0006493 [protein O-linked glycosylation]
GO:0006915 [apoptotic process]
GO:0007165 [signal transduction]
GO:0007584 [response to nutrient]
GO:0008047 [enzyme activator activity]
GO:0008134 [transcription factor binding]
GO:0008375 [acetylglucosaminyltransferase activity]
GO:0010628 [positive regulation of gene expression]
GO:0010801 [negative regulation of peptidyl-threonine phosphorylation]
GO:0016262 [protein N-acetylglucosaminyltransferase activity]
GO:0019904 [protein domain specific binding]
GO:0030854 [positive regulation of granulocyte differentiation]
GO:0030900 [forebrain development]
GO:0031397 [negative regulation of protein ubiquitination]
GO:0032868 [response to insulin]
GO:0032869 [cellular response to insulin stimulus]
GO:0032922 [circadian regulation of gene expression]
GO:0033137 [negative regulation of peptidyl-serine phosphorylation]
GO:0035020 [regulation of Rac protein signal transduction]
GO:0042277 [peptide binding]
GO:0042588 [zymogen granule]
GO:0043005 [neuron projection]
GO:0043025 [neuronal cell body]
GO:0043085 [positive regulation of catalytic activity]
GO:0043981 [histone H4-K5 acetylation]
GO:0043982 [histone H4-K8 acetylation]
GO:0043984 [histone H4-K16 acetylation]
GO:0043995 [histone acetyltransferase activity (H4-K5 specific)]
GO:0043996 [histone acetyltransferase activity (H4-K8 specific)]
GO:0045793 [positive regulation of cell size]
GO:0045862 [positive regulation of proteolysis]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0046626 [regulation of insulin receptor signaling pathway]
GO:0046972 [histone acetyltransferase activity (H4-K16 specific)]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048029 [monosaccharide binding]
GO:0048312 [intracellular distribution of mitochondria]
GO:0051571 [positive regulation of histone H3-K4 methylation]
GO:0060548 [negative regulation of cell death]
GO:0061087 [positive regulation of histone H3-K27 methylation]
GO:0070207 [protein homotrimerization]
GO:0070208 [protein heterotrimerization]
GO:0070688 [MLL5-L complex]
GO:0071222 [cellular response to lipopolysaccharide]
GO:0071300 [cellular response to retinoic acid]
GO:0071333 [cellular response to glucose stimulus]
GO:0080182 [histone H3-K4 trimethylation]
GO:0090315 [negative regulation of protein targeting to membrane]
GO:0090526 [regulation of gluconeogenesis involved in cellular glucose homeostasis]
GO:0097237 [cellular response to toxic substance]
GO:0097363 [protein O-GlcNAc transferase activity]
GO:1900038 [negative regulation of cellular response to hypoxia]
GO:1900182 [positive regulation of protein localization to nucleus]
GO:1903428 [positive regulation of reactive oxygen species biosynthetic process]
Show all
1046 aa
116.9 kDa
No 0
OGT-002
ENSP00000362805
ENST00000373701
O15294 [Direct mapping]
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000123 [histone acetyltransferase complex]
GO:0005515 [protein binding]
GO:0005547 [phosphatidylinositol-3,4,5-trisphosphate binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005815 [microtubule organizing center]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006110 [regulation of glycolytic process]
GO:0006325 [chromatin organization]
GO:0006493 [protein O-linked glycosylation]
GO:0006915 [apoptotic process]
GO:0007165 [signal transduction]
GO:0007584 [response to nutrient]
GO:0008047 [enzyme activator activity]
GO:0008375 [acetylglucosaminyltransferase activity]
GO:0016262 [protein N-acetylglucosaminyltransferase activity]
GO:0030854 [positive regulation of granulocyte differentiation]
GO:0031397 [negative regulation of protein ubiquitination]
GO:0032868 [response to insulin]
GO:0032922 [circadian regulation of gene expression]
GO:0035020 [regulation of Rac protein signal transduction]
GO:0043085 [positive regulation of catalytic activity]
GO:0043981 [histone H4-K5 acetylation]
GO:0043982 [histone H4-K8 acetylation]
GO:0043984 [histone H4-K16 acetylation]
GO:0043995 [histone acetyltransferase activity (H4-K5 specific)]
GO:0043996 [histone acetyltransferase activity (H4-K8 specific)]
GO:0045862 [positive regulation of proteolysis]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0046626 [regulation of insulin receptor signaling pathway]
GO:0046972 [histone acetyltransferase activity (H4-K16 specific)]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0051571 [positive regulation of histone H3-K4 methylation]
GO:0061087 [positive regulation of histone H3-K27 methylation]
GO:0070688 [MLL5-L complex]
GO:0071300 [cellular response to retinoic acid]
GO:0080182 [histone H3-K4 trimethylation]
GO:0090526 [regulation of gluconeogenesis involved in cellular glucose homeostasis]
GO:0097363 [protein O-GlcNAc transferase activity]
Show all
1036 aa
115.7 kDa
No 0
OGT-011
ENSP00000399729
ENST00000444774
C9JZL3 [Direct mapping]
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
156 aa
17.6 kDa
No 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.