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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytosol & endoplasmic reticulum and GFP expression in cytosol & endoplasmic reticulum.
Antibody dilution
1:5
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
Antibody dilution
1:5
1:37
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011311
Antibody CAB010339
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011311
Antibody CAB010339
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.6, 24.5, 23.1, 20.4, 18.6
24.6, 23.1, 20.4, 20.2, 18.6, 14.6, 12.3, 10, 3.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA011311
Antibody CAB010339
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
