Fields »
ABOUT
HELP
BLOG
CDKN2A

ANTIBODY INFORMATION
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Lab Vision/NeoMarkers
   Santa Cruz Biotechnology
  

Product name

 HPA047838   NCL-p16-432   RB-1554   sc-56330  

Host species

 Rabbit   Mouse   Rabbit   Mouse  

Clonality

 pAb   mAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known   Protein A/G  

Other gene match

        

Released in version

 12   1   1   4  

References

        

Proper citation

 Atlas Antibodies Cat#HPA047838, RRID:AB_2680174       Santa Cruz Biotechnology Cat#sc-56330, RRID:AB_785018  

VALIDATION SUMMARY
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  
Immunocytochemistry

Supported        
Immunohistochemistry

  Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Standard validation

 Supported        

       

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.        

Antibody dilution

 1:54        

Literature conformity

 The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Standard validation

   Uncertain   Uncertain   Uncertain  

       

Figure description

   Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human lymph node shows moderate nuclear positivity in a subset of cells.   Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.  

Expression

   RNA: detected in 21 tissues
Protein: detected in 7 cell types		   RNA: detected in 21 tissues
Protein: detected in 66 cell types		   RNA: detected in 21 tissues
Protein: detected in 25 cell types		  

Retrieval

   HIER pH9   HIER pH6   HIER pH6  

Antibody dilution

   1:100   1:200   1:200  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 18.5, 13.9   18.5, 17.9, 16.5, 15, 13.9, 12.7, 11.2, 11.1   18.5, 17.9, 16.5, 15, 13.9, 12.7, 11.2, 11.1   18.5, 17.9, 16.5, 15, 13.9, 12.7, 11.2, 11.1  

Antibody dilution

 1:270   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:2150        

ANTIGEN INFORMATION
 
 

Antibody HPA047838

  

Antibody CAB000093

  

Antibody CAB000445

  

Antibody CAB018232

  

Antigen

 Recombinant protein fragment   Not known   Not known   Not known  

Length (aa)

 68        

Antigen sequence

 
LVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRL
GQQPLPRRPGHDDGQRPS
       

Matching transcripts

 CDKN2A-004 - ENSP00000462950 [100%]
CDKN2A-013 - ENSP00000432664 [100%]
CDKN2A-201 - ENSP00000355153 [100%]
       

Other gene match

        
ANTIGEN VIEW
CDKN2A-004
CDKN2A-013
CDKN2A-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.