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Immunofluorescent staining of human cell line MCF7 shows localization to cell junctions.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & cell junctions.
Antibody dilution
1:23
1:65
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038387
Antibody HPA038388
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human placenta shows strong membranous positivity.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 31 tissues Protein: detected in 40 cell types
RNA: detected in 31 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:250
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038387
Antibody HPA038388
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408963)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408963)
Target mass (kDa)
13.9
13.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA038387
Antibody HPA038388
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
