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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.
Antibody dilution
1:37
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037597
Antibody HPA037598
Standard validation
Supported
Figure description
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 36 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037597
Antibody HPA037598
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406579)
Target mass (kDa)
65.5, 36
65.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037597
Antibody HPA037598
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
