Fields »
ABOUT
HELP
BLOG
ADGRL3

ANTIBODY INFORMATION
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA015027   HPA015762   AB9193  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 5   4   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA015027, RRID:AB_1853243   Atlas Antibodies Cat#HPA015762, RRID:AB_1853245    

VALIDATION SUMMARY
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells while islet cells were moderately stained.  

Expression

 RNA: detected in 28 tissues
Protein: detected in 69 cell types		   RNA: detected in 28 tissues
Protein: detected in 55 cell types		   RNA: detected in 28 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:15   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 176.1, 174.9, 170.4, 169.1, 168, 164.6, 150.8, 150.4, 149.6, 146.2, 145.1, 143.8, 142.7, 139.3, 138.1   176.1, 174.9, 170.4, 169.1, 168, 164.6, 150.8, 150.4, 149.6, 146.2, 145.1, 143.8, 142.7, 139.3, 138.1, 102.3   176.1, 174.9, 170.4, 169.1, 168, 164.6, 150.8, 150.4, 149.6, 146.2, 145.1, 143.8, 142.7, 139.3, 138.1, 102.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA015027

  

Antibody HPA015762

  

Antibody CAB022698

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 150   91    

Antigen sequence

 
DNLLYVWNNYHVVKYSLDFGPLDSRSGQAHHGQVSYISPPIHLDSELERP
SVKDISTTGPLGMGSTTTSTTLRTTTLSPGRSTTPSVSGRRNRSTSTPSP
AVEVLDDMTTHLPSASSQIPALEESCEAVEAREIMWFKTRQGQIAKQPCP
  
LCLAPDGIWDPQGPDLSNCSSPWVNHITQKLKSGETAANIARELAEQTRN
HLNAGDITYSVRAMDQLVGLLDVQLRNLTPGGKDSAARSLN
  

											 
																				

																		

										
											
Matching transcripts

												
																					ADGRL3-001 - ENSP00000423388 [100%] 
ADGRL3-003 - ENSP00000422533 [100%] 
ADGRL3-004 - ENSP00000421476 [100%] 
ADGRL3-006 - ENSP00000425201 [100%] 
ADGRL3-007 - ENSP00000421627 [100%] 
ADGRL3-008 - ENSP00000420931 [100%] 
ADGRL3-009 - ENSP00000425884 [100%] 
ADGRL3-010 - ENSP00000424258 [100%] 
ADGRL3-011 - ENSP00000424030 [100%] 
ADGRL3-012 - ENSP00000423434 [100%] 
ADGRL3-013 - ENSP00000423787 [100%] 
ADGRL3-014 - ENSP00000425033 [100%] 
ADGRL3-015 - ENSP00000424120 [100%] 
ADGRL3-016 - ENSP00000421372 [100%] 
ADGRL3-201 - ENSP00000439831 [100%] 

											 
																						ADGRL3-001 - ENSP00000423388 [100%] 
ADGRL3-002 - ENSP00000424402 [100%] 
ADGRL3-003 - ENSP00000422533 [100%] 
ADGRL3-004 - ENSP00000421476 [100%] 
ADGRL3-006 - ENSP00000425201 [100%] 
ADGRL3-007 - ENSP00000421627 [100%] 
ADGRL3-008 - ENSP00000420931 [100%] 
ADGRL3-009 - ENSP00000425884 [100%] 
ADGRL3-010 - ENSP00000424258 [100%] 
ADGRL3-011 - ENSP00000424030 [100%] 
ADGRL3-012 - ENSP00000423434 [100%] 
ADGRL3-013 - ENSP00000423787 [100%] 
ADGRL3-014 - ENSP00000425033 [100%] 
ADGRL3-015 - ENSP00000424120 [100%] 
ADGRL3-016 - ENSP00000421372 [100%] 
ADGRL3-201 - ENSP00000439831 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

					«					
						

							

								

									
ADGRL3-001
ADGRL3-002
ADGRL3-003
ADGRL3-004
ADGRL3-006
ADGRL3-007
ADGRL3-008
ADGRL3-009
ADGRL3-010
ADGRL3-011
ADGRL3-012
ADGRL3-013
ADGRL3-014
ADGRL3-015
ADGRL3-016
ADGRL3-201
								

							

						

							
					»				

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.