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GPR158

ANTIBODY INFORMATION
 
 

Antibody HPA013185

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA013185  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA013185, RRID:AB_2113957  

VALIDATION SUMMARY
 
 

Antibody HPA013185

  
Immunocytochemistry

Uncertain  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA013185

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:29  

Literature conformity

 The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013185

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:25  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013185

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 135.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA013185

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA013185

  

Antigen

 Recombinant protein fragment  

Length (aa)

 139  

Antigen sequence

 
LGLAGKTQTAGVEERTKSQKPLPKDKETNRNHSNSDNTETKDPAPQNSNP
AEEPRKPQKSGIMKQQRVNPTTANSDLNPGTTQMKDNFDIGEVCPWEVYD
LTPGPVPSESKVQKHVSIVASEMEKNPTFSLKEKSHHKP
 

Matching transcripts

 GPR158-001 - ENSP00000365529 [100%]
 

Other gene match

  
ANTIGEN VIEW
GPR158-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.