We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA061035
Antibody CAB002008
Antibody CAB002009
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human lateral ventricle shows weak nuclear positivity in glial cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 23 cell types
RNA: detected in 36 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA061035
Antibody CAB002008
Antibody CAB002009
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
52.8, 14, 13.9, 13, 11.9, 7.7, 7.4
54.4, 52.8, 15.2, 14, 13.9, 13, 11.9, 7.7, 7.4
54.4, 52.8, 15.2, 14, 13.9, 13, 11.9, 7.7, 7.4
Antibody dilution
1:170
1:500
1:500
PROTEIN ARRAY
Antibody HPA061035
Antibody CAB002008
Antibody CAB002009
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
