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Immunohistochemical staining of human kidney shows nuclear and cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042339
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407684)
Target mass (kDa)
11.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA042339
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
