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ANTIBODY INFORMATION
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA007641   AB3897  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 3   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA007641, RRID:AB_1078845    

VALIDATION SUMMARY
 
 

Antibody HPA007641

  

Antibody CAB022464

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.    

Antibody dilution

 1:30    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 57 cell types		   RNA: detected in 33 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:4000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 94.6   94.6, 51.6, 47, 19  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA007641

  

Antibody CAB022464

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 132    

Antigen sequence

 
QVMLKTLAEELMQTQQMLLNKEEAVLELEKRIEESSETCEKKSDIVLLLS
QKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQENDGKEPPPVVNYEED
ARSVTSMERKERLSKFESIRHSIAGIIRSPKS
  

											 
																				

																		

										
											
Matching transcripts

												
																					FER-001 - ENSP00000281092 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FER-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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