Expression of ASAP2 in cancer - Summary

GENERAL INFORMATION
Gene name	ASAP2
Gene description	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Protein class	Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Mixed
HPA (normal tissue):	Tissue enhanced (testis)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and nuclear expression in several tissues, most abundant in testis and gastrointestinal tract.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data.
Reliability score	Approved based on 1 antibody. CAB018615
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PROGNOSTIC SUMMARY
Prognostic marker in endometrial cancer (unfavourable) and pancreatic cancer (unfavourable).
Endometrial cancer p<0.001 Pancreatic cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

CAB018615

GENE INFORMATION
Gene name	ASAP2 (HGNC Symbol)
Synonyms	CENTB3, DDEF2, KIAA0400, PAP, SHAG1
Description	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (HGNC Symbol)
Entrez gene summary	This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Chromosome	2
Cytoband	p25.1
Chromosome location (bp)	9206765 - 9405683
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000151693 (version 83.38)
Entrez gene	8853
UniProt	O43150 (UniProt - Evidence at protein level)
neXtProt	NX_O43150
Antibodypedia	ASAP2 antibodies

PROTEIN BROWSER

ASAP2-001	ASAP2-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ASAP2-001 ENSP00000281419 ENST00000281419	O43150 [Direct mapping] Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005096 [GTPase activator activity] GO:0005515 [protein binding] GO:0005886 [plasma membrane] GO:0008047 [enzyme activator activity] GO:0032580 [Golgi cisterna membrane] GO:0043085 [positive regulation of catalytic activity] GO:0043547 [positive regulation of GTPase activity] GO:0046872 [metal ion binding] Show all	1006 aa 111.7 kDa	No	0
ASAP2-201 ENSP00000316404 ENST00000315273	O43150 [Direct mapping] Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005096 [GTPase activator activity] GO:0005515 [protein binding] GO:0005886 [plasma membrane] GO:0008047 [enzyme activator activity] GO:0032580 [Golgi cisterna membrane] GO:0043085 [positive regulation of catalytic activity] GO:0043547 [positive regulation of GTPase activity] GO:0046872 [metal ion binding] Show all	961 aa 107 kDa	No	0