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BMP6

ANTIBODY INFORMATION
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA062683   MAB1048  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 16   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA062683, RRID:AB_2684832    

VALIDATION SUMMARY
 
 

Antibody HPA062683

  

Antibody CAB002773

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:52   1:8  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

   RNA: detected in 30 tissues
Protein: detected in 63 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:30  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 57.2   57.2  

Antibody dilution

 1:170   1:500  

PROTEIN ARRAY
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2100    

ANTIGEN INFORMATION
 
 

Antibody HPA062683

  

Antibody CAB002773

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 56    

Antigen sequence

 
FKVSEVHVRTTRSASSRRRQQSRNRSTQSQDVARVSSASDYNSSELKTAC
RKHELY
  

											 
																				

																		

										
											
Matching transcripts

												
																					BMP6-001 - ENSP00000283147 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BMP6-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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