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Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane & vesicles.
Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear membrane & vesicles.
Antibody dilution
1:61
1:53
Literature conformity
The subcellular location is supported by literature.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018437
Antibody HPA023960
Antibody HPA049497
Antibody HPA051675
Antibody HPA067564
Antibody CAB034063
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA023960 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA018437 across 72 cell types.
Standard validation
Supported
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in trophoblastic cells.
Immunohistochemical staining of human stomach shows strong nuclear membrane positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear membrane positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
RNA: detected in 37 tissues Protein: detected in 28 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:400
1:80
1:100
1:20
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018437
Antibody HPA023960
Antibody HPA049497
Antibody HPA051675
Antibody HPA067564
Antibody CAB034063
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
