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Antibody staining overlaps with antibody HPA062287.
Validated
Antibody staining overlaps with antibody HPA041353.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.
Antibody dilution
1:24
1:54
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041353
Antibody HPA062287
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041353
Antibody HPA062287
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412311)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
33.3, 20.8
33.3, 21.3, 14
Antibody dilution
1:250
1:180
PROTEIN ARRAY
Antibody HPA041353
Antibody HPA062287
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
