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Antibody staining overlaps with antibody HPA059642.
Validated
Antibody staining overlaps with antibody HPA059054.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Antibody dilution
1:44
1:66
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019215
Antibody HPA059054
Antibody HPA059642
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in a subset of hematopoietic cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 5 cell types
RNA: detected in 37 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:350
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019215
Antibody HPA059054
Antibody HPA059642
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38.9
38.9, 35.1
38.9, 35.1, 10.6
Antibody dilution
1:250
1:150
1:220
PROTEIN ARRAY
Antibody HPA019215
Antibody HPA059054
Antibody HPA059642
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.