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Antibody staining overlaps with antibody HPA067180.
Validated
Antibody staining overlaps with antibody HPA064878.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Antibody dilution
1:137
1:58
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA064878
Antibody HPA067180
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
184.3
184.3
Antibody dilution
1:460
1:190
PROTEIN ARRAY
Antibody HPA064878
Antibody HPA067180
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:5500
1:2350
ANTIGEN INFORMATION
Antibody HPA064878
Antibody HPA067180
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
61
Antigen sequence
LYFDKILPYGDERHYSPLFFLNSSSCFQHQRTNAKVIEKEIDAEHPSDDY
F