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ANTIBODY INFORMATION
 
 

Antibody HPA007042

  

Antibody CAB022682

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA007042   MAB1553  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Serum  

Other gene match

    

Released in version

 3   5  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA007042, RRID:AB_1080292    

VALIDATION SUMMARY
 
 

Antibody HPA007042

  

Antibody CAB022682

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007042

  

Antibody CAB022682

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 57 cell types		   RNA: detected in 19 tissues
Protein: detected in 26 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:120  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007042

  

Antibody CAB022682

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 3994.6, 3816, 3713.5, 3013.9, 3006.7, 2992.9, 631.6   3994.6, 3816, 3713.5, 3013.9, 3006.7, 2992.9, 631.6, 109.2, 41.5, 39.9, 28.2, 26.7, 23.2, 20, 5.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007042

  

Antibody CAB022682

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA007042

  

Antibody CAB022682

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 134    

Antigen sequence

 
GTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTE
EQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWKKSGVP
LTTGYRYKVSYNKQTGECKLVISMTFADDAGEYT
  

											 
																				

																		

										
											
Matching transcripts

												
																					TTN-002 - ENSP00000343764 [100%] 
TTN-003 - ENSP00000434586 [100%] 
TTN-010 - ENSP00000354117 [100%] 
TTN-018 - ENSP00000467141 [100%] 
TTN-019 - ENSP00000465570 [100%] 
TTN-201 - ENSP00000340554 [100%] 
TTN-202 - ENSP00000352154 [100%] 
TTN-203 - ENSP00000483597 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TTN-002
TTN-003
TTN-010
TTN-018
TTN-019
TTN-201
TTN-202
TTN-203
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA007042

												
										 
																				
											
											
											
Antibody CAB022682

												
										 
																		

																	

										
											
1.

												
																						Generation and characterization of functional cardiomyocytes derived from human T cell-derived induced pluripotent stem cells

	Seki T et al

	PLoS One 2014;9(1):e85645

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Differential Protein Expression in Congenital and Acquired Cholesteatomas

	Shin SH et al

	PLoS One 2015;10(9):e0137011

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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