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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA024780
Standard validation
Approved
Figure description
Immunohistochemical staining of human placenta shows distinct cytoplasmic and membranous positivity in trophoblasts.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA024780
Standard validation
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405618)
Target mass (kDa)
83.8, 21.7, 13
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA024780
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
