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EEF1A1

ANTIBODY INFORMATION
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051759   HPA053862   HPA056990  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 EEF1A2 - ENSG00000101210 [100%]
   EEF1A2 - ENSG00000101210 [100%]
   EEF1A2 - ENSG00000101210 [100%]
  

Released in version

 12   12   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA051759, RRID:AB_2681595     Atlas Antibodies Cat#HPA056990, RRID:AB_2683302  

VALIDATION SUMMARY
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:28   1:23    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.     Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		     RNA: detected in 37 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:50     1:1000  

Literature conformity

 Consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.5, 50.1, 47.9, 46.4   50.5, 50.1, 47.9, 46.4   50.5, 50.1, 47.9, 46.4  

Antibody dilution

 1:140   1:120   1:340  

PROTEIN ARRAY
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1100   1:950   1:4100  

ANTIGEN INFORMATION
 
 

Antibody HPA051759

  

Antibody HPA053862

  

Antibody HPA056990

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 45   32   49  

Antigen sequence

 
LLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGIL
  
PVIDCHTAHIACKFAELKEKIDRRSGKKLEDN
  
PGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRR
 

Matching transcripts

 EEF1A1-001 - ENSP00000339063 [96%]
EEF1A1-004 - ENSP00000330054 [96%]
EEF1A1-009 - ENSP00000339053 [96%]
EEF1A1-201 - ENSP00000479609 [96%]
EEF1A1-202 - ENSP00000479055 [80%]
  EEF1A1-001 - ENSP00000339063 [94%]
EEF1A1-004 - ENSP00000330054 [94%]
EEF1A1-009 - ENSP00000339053 [94%]
EEF1A1-201 - ENSP00000479609 [94%]
EEF1A1-202 - ENSP00000479055 [94%]
  EEF1A1-001 - ENSP00000339063 [96%]
EEF1A1-004 - ENSP00000330054 [96%]
EEF1A1-009 - ENSP00000339053 [96%]
EEF1A1-201 - ENSP00000479609 [96%]
EEF1A1-202 - ENSP00000479055 [96%]
 

Other gene match

 EEF1A2 - ENSG00000101210 [100%]
   EEF1A2 - ENSG00000101210 [100%]
   EEF1A2 - ENSG00000101210 [100%]
  
ANTIGEN VIEW
EEF1A1-001
EEF1A1-004
EEF1A1-009
EEF1A1-201
EEF1A1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.