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RPUSD3

ANTIBODY INFORMATION
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037551   HPA037552  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA037551, RRID:AB_2675538   Atlas Antibodies Cat#HPA037552, RRID:AB_10672600  

VALIDATION SUMMARY
 
 

Antibody HPA037551

  

Antibody HPA037552

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.    

Antibody dilution

 1:90    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 22 cell types		   RNA: detected in 37 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.5, 36.9   38.5, 29.3, 26.4, 22.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037551

  

Antibody HPA037552

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   81  

Antigen sequence

 
VHMVLQLCPVLGDHMYSARVGTVLGQRFLLPAENNKPQRQVLDEALLRRL
HLTPSQAAQLPLHLHLHRLLLPGTR
  
VVDRKGPLVTLNKPQGLPVTGKPGELTLFSVLPELSQSLGLREQELQVVR
ASGKESSGLVLLSSCPQTASRLQKYFTHARR
 

Matching transcripts

 RPUSD3-001 - ENSP00000373331 [100%]
RPUSD3-002 - ENSP00000398921 [100%]
  RPUSD3-001 - ENSP00000373331 [100%]
RPUSD3-009 - ENSP00000400397 [100%]
RPUSD3-010 - ENSP00000408693 [100%]
RPUSD3-011 - ENSP00000415616 [100%]
 

Other gene match

    
ANTIGEN VIEW
RPUSD3-001
RPUSD3-002
RPUSD3-009
RPUSD3-010
RPUSD3-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.