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ANTIBODY INFORMATION
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004471   HPA073252   A4502   AMAb90901   AMAb90904  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   msAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 3   16   2   13   14  

References

          

Proper citation

 Atlas Antibodies Cat#HPA004471, RRID:AB_1078431   Atlas Antibodies Cat#HPA073252, RRID:AB_2686589   Dako Cat#A4502, RRID:AB_2335702   Atlas Antibodies Cat#AMAb90901, RRID:AB_2665717   Atlas Antibodies Cat#AMAb90904, RRID:AB_2665718  

VALIDATION SUMMARY
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  
Immunocytochemistry

  Supported        
Immunohistochemistry


Validated
    

Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Standard validation

   Supported        

         

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.        

Antibody dilution

   1:156        

Literature conformity

   The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported     Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.     Immunohistochemical staining of human testis shows cytoplasmic positivity in a fraction of cells in seminiferous ducts.   Immunohistochemical staining of human small intestine shows distinct positivity in peripheral leukocytes.   Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in basket cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 19 cell types		     RNA: detected in 34 tissues
Protein: detected in 8 cell types		   RNA: detected in 34 tissues
Protein: detected in 6 cell types		   RNA: detected in 34 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:40     1:300   1:1000   1:350  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

         

Figure description

          

Target mass (kDa)

 109.9, 109.4   109.9, 109.4   109.9, 109.4   109.9, 109.4   109.9, 109.4  

Antibody dilution

 1:250   1:520   1:500   1:1000   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:6250        

ANTIGEN INFORMATION
 
 

Antibody HPA004471

  

Antibody HPA073252

  

Antibody CAB003288

  

Antibody CAB068253

  

Antibody CAB072867

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Recombinant protein   Recombinant protein  

Length (aa)

 141   81        

Antigen sequence

 
VGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTN
KHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLK
GCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQ
  
RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVP
TKADKRRSVRIGSYIERDVTPAIMEDDELAL
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					KIT-001 - ENSP00000288135 [100%] 
KIT-002 - ENSP00000390987 [100%] 

											 
																						KIT-001 - ENSP00000288135 [100%] 
KIT-002 - ENSP00000390987 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KIT-001
KIT-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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