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Immunohistochemical staining of human testis shows strong nuclear positivity in a subset of cells.
Immunohistochemical staining of human testis shows moderate nuclear positivity in a subset of cells.
Expression
RNA: detected in 20 tissues Protein: detected in 44 cell types
RNA: detected in 20 tissues Protein: detected in 31 cell types
Retrieval
HIER pH9
HIER pH6
Antibody dilution
1:100
1:1000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000168
Antibody HPA020464
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407544)
Target mass (kDa)
79, 47.4
79, 47.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA000168
Antibody HPA020464
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
