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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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BRAF
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

BRAF

Gene description

B-Raf proto-oncogene, serine/threonine kinase

Protein class

Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA001328 , CAB004552
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA001328
CAB004552

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most malignant tissues showed moderate cytoplasmic staining. Strong immunoreactivity was observed in few cases of prostate-, breast- and thyroid cancer.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most malignancies showed moderate to strong cytoplasmic positivity.

GENE INFORMATION

Gene name

BRAF (HGNC Symbol)

Synonyms

BRAF1

Description

B-Raf proto-oncogene, serine/threonine kinase (HGNC Symbol)

Entrez gene summary

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

Chromosome

7

Cytoband

q34

Chromosome location (bp)

140719327 - 140924764

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000157764 (version 83.38)

Entrez gene

673

UniProt

P15056 (UniProt - Evidence at protein level)

neXtProt

NX_P15056

Antibodypedia

BRAF antibodies

PROTEIN BROWSER

BRAF-001
BRAF-003

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

BRAF-001
ENSP00000288602
ENST00000288602
P15056 [Direct mapping]
Serine/threonine-protein kinase B-raf
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   TKL Ser/Thr protein kinases
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Other Mutations
   COSMIC Missense Mutations
   COSMIC Translocations
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0002318 [myeloid progenitor cell differentiation]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0004709 [MAP kinase kinase kinase activity]
GO:0005057 [receptor signaling protein activity]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006468 [protein phosphorylation]
GO:0007165 [signal transduction]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007268 [synaptic transmission]
GO:0007411 [axon guidance]
GO:0008286 [insulin receptor signaling pathway]
GO:0008542 [visual learning]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0009887 [organ morphogenesis]
GO:0010628 [positive regulation of gene expression]
GO:0010764 [negative regulation of fibroblast migration]
GO:0015758 [glucose transport]
GO:0016020 [membrane]
GO:0030154 [cell differentiation]
GO:0030878 [thyroid gland development]
GO:0031434 [mitogen-activated protein kinase kinase binding]
GO:0033138 [positive regulation of peptidyl-serine phosphorylation]
GO:0035019 [somatic stem cell population maintenance]
GO:0035556 [intracellular signal transduction]
GO:0035690 [cellular response to drug]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042127 [regulation of cell proliferation]
GO:0042802 [identical protein binding]
GO:0043005 [neuron projection]
GO:0043066 [negative regulation of apoptotic process]
GO:0043367 [CD4-positive, alpha-beta T cell differentiation]
GO:0043368 [positive T cell selection]
GO:0043434 [response to peptide hormone]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0044297 [cell body]
GO:0045087 [innate immune response]
GO:0046632 [alpha-beta T cell differentiation]
GO:0046982 [protein heterodimerization activity]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048538 [thymus development]
GO:0048679 [regulation of axon regeneration]
GO:0048680 [positive regulation of axon regeneration]
GO:0050772 [positive regulation of axonogenesis]
GO:0051291 [protein heterooligomerization]
GO:0051496 [positive regulation of stress fiber assembly]
GO:0051591 [response to cAMP]
GO:0060291 [long-term synaptic potentiation]
GO:0060323 [head morphogenesis]
GO:0060324 [face development]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0071277 [cellular response to calcium ion]
GO:0090150 [establishment of protein localization to membrane]
GO:1900026 [positive regulation of substrate adhesion-dependent cell spreading]
GO:2000301 [negative regulation of synaptic vesicle exocytosis]
GO:2000352 [negative regulation of endothelial cell apoptotic process]
Show all
766 aa
84.4 kDa
No 0
BRAF-003
ENSP00000419060
ENST00000496384
H7C560 [Direct mapping]
Serine/threonine-protein kinase B-raf
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Other Mutations
   COSMIC Missense Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
Show all
375 aa
41.8 kDa
No 0

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The Human Protein Atlas project is funded
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