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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ACAN

Gene description

Aggrecan

Protein class

Predicted intracellular proteins
Predicted secreted proteins

Predicted localization

Intracellular,Secreted

Number of transcripts

8
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Mixed
HPA (cell line):Cell line enriched (WM-115)
HPA (normal tissue):Tissue enhanced (seminal vesicle)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

High expression in chondrocytes and cartilage.

ANTIBODY IHC RELIABILITY

Data reliability
description

Pending additional tissues.

Reliability score

Supported based on 3 antibodies.
HPA038241 , HPA038242 , CAB016377
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable).
Renal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Mixed

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA038241
HPA038242
CAB016377

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Renal cancers showed moderate to strong membranous and cytoplasmic immunoreactivity. Few cases of breast cancers showed moderate staining. A subset of tumor cells in few cases of cervix, endometrial, lung and skin cancers displayed moderate cytoplasmic positivity. A single case of duct carcinoma of breast exhibited strong nucleolar staining. Remaining cancer tissues were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A single case of melanoma and fraction of cells in a rare case of urothelial cancer displayed weak to moderate cytoplasmic positivity. Remaining cancer tissues were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were in general negative. Cases of hepatocellular carcinomas displayed moderate positivity.

GENE INFORMATION

Gene name

ACAN (HGNC Symbol)

Synonyms

AGC1, CSPG1, CSPGCP, MSK16

Description

Aggrecan (HGNC Symbol)

Entrez gene summary

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Chromosome

15

Cytoband

q26.1

Chromosome location (bp)

88803443 - 88875354

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000157766 (version 83.38)

Entrez gene

176

UniProt

neXtProt

Antibodypedia

ACAN antibodies

PROTEIN BROWSER

ACAN-001
ACAN-003
ACAN-004
ACAN-005
ACAN-006
ACAN-008
ACAN-201
ACAN-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ACAN-001
ENSP00000453003
ENST00000558207
Q6PID9 [Direct mapping]
Aggrecan; Aggrecan core protein
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Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
721 aa
79.6 kDa
Yes 0
ACAN-003
ENSP00000452682
ENST00000558704
H0YK70 [Direct mapping]
Aggrecan core protein
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
156 aa
17.3 kDa
No 0
ACAN-004
ENSP00000341615
ENST00000352105
E7ENV9 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2431 aa
250.4 kDa
Yes 0
ACAN-005
ENSP00000453581
ENST00000560601
H0YMF1 [Direct mapping]
Aggrecan core protein
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
226 aa
24.2 kDa
No 0
ACAN-006
ENSP00000453342
ENST00000561243
E7EX88 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2530 aa
261.3 kDa
Yes 0
ACAN-008
ENSP00000453499
ENST00000559004
H0YM81 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2492 aa
257.4 kDa
Yes 0
ACAN-201
ENSP00000387356
ENST00000439576
E7EX88 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0001502 [cartilage condensation]
GO:0002063 [chondrocyte development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0005604 [basement membrane]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
GO:0007507 [heart development]
GO:0030166 [proteoglycan biosynthetic process]
GO:0030199 [collagen fibril organization]
Show all
2530 aa
261.3 kDa
Yes 0
ACAN-202
ENSP00000484456
ENST00000617301
A0A087X1T7 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2473 aa
255.6 kDa
Yes 0

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The Human Protein Atlas project is funded
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