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RER1

ANTIBODY INFORMATION
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA051400   R4407  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051400, RRID:AB_2681469   Sigma-Aldrich Cat#R4407, RRID:AB_2179604  

VALIDATION SUMMARY
 
 

Antibody HPA051400

  

Antibody CAB037240

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.  

Antibody dilution

 1:33   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1500  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 24.8, 23, 21, 20.8, 18.4   24.8, 23, 21, 20.8, 18.4, 13.4  

Antibody dilution

 1:160   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1300    

ANTIGEN INFORMATION
 
 

Antibody HPA051400

  

Antibody CAB037240

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 38    

Antigen sequence

 
LSPKVDPSLMEDSDDGPSLPTKQNEEFRPFIRRLPEFK
   

Matching transcripts

 RER1-001 - ENSP00000475168 [100%]
RER1-002 - ENSP00000367773 [100%]
RER1-003 - ENSP00000464222 [100%]
RER1-006 - ENSP00000417000 [100%]
RER1-008 - ENSP00000302088 [100%]
RER1-202 - ENSP00000487501 [100%]
   

Other gene match

    
ANTIGEN VIEW
RER1-001
RER1-002
RER1-003
RER1-006
RER1-008
RER1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.