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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
Expression
RNA: detected in 32 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA061689
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73, 70.8, 70.7, 68.6, 67.8, 60.8
Antibody dilution
1:130
PROTEIN ARRAY
Antibody HPA061689
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1550
ANTIGEN INFORMATION
Antibody HPA061689
Antigen
Recombinant protein fragment
Length (aa)
53
Antigen sequence
LQSDSELGRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATHQSEEDEE
DEE