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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:69
1:95
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019073
Antibody HPA019075
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity, with a granular pattern in glandular cells.
Expression
RNA: detected in 27 tissues Protein: detected in 6 cell types
RNA: detected in 27 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:1200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019073
Antibody HPA019075
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409746)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409746)
Target mass (kDa)
59.3
59.3
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA019073
Antibody HPA019075
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA019073
Antibody HPA019075
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
66
Antigen sequence
AEEAALGPTEPAEGLSAPSLSPHCCPCRARLAFRNLGALLPRLHQLCCRM
P
