Fields »
ABOUT
HELP
BLOG
MED27

GENERAL INFORMATION

Gene name

 MED27

Gene description

 Mediator complex subunit 27

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 General nuclear and cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Approved based on 1 antibody.
HPA007002
SHOW MORE

PROGNOSTIC SUMMARY
Prognostic marker in cervical cancer (favourable).
Cervical cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA007002

GENE INFORMATION

Gene name

 MED27 (HGNC Symbol)

Synonyms

 CRSP34, CRSP8, MED3, TRAP37

Description

 Mediator complex subunit 27 (HGNC Symbol)

Entrez gene summary

 The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Chromosome

 9

Cytoband

 q34.13

Chromosome location (bp)

 131860107 - 132079908

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000160563 (version 83.38)

Entrez gene

 9442

UniProt

 Q6P2C8 (UniProt - Evidence at protein level)

neXtProt

 NX_Q6P2C8

Antibodypedia

 MED27 antibodies

PROTEIN BROWSER
MED27-001
MED27-002
MED27-003

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
MED27-001
ENSP00000292035
ENST00000292035
Q6P2C8 [Direct mapping]
Mediator of RNA polymerase II transcription subunit 27
A0A024R8B7 [Target identity:100%; Query identity:100%]
Cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa, isoform CRA_b
Show all
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0003713 [transcription coactivator activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006367 [transcription initiation from RNA polymerase II promoter]
GO:0010467 [gene expression]
GO:0016567 [protein ubiquitination]
GO:0016592 [mediator complex]
GO:0019827 [stem cell population maintenance]
GO:0061630 [ubiquitin protein ligase activity]
Show all
 311 aa
35.4 kDa 		No 0
MED27-002
ENSP00000349530
ENST00000357028
Q6P2C8 [Direct mapping]
Mediator of RNA polymerase II transcription subunit 27
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003713 [transcription coactivator activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006367 [transcription initiation from RNA polymerase II promoter]
GO:0010467 [gene expression]
Show all
 275 aa
31.4 kDa 		No 0
MED27-003
ENSP00000477136
ENST00000474263
Q6P2C8 [Direct mapping]
Mediator of RNA polymerase II transcription subunit 27
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003713 [transcription coactivator activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006357 [regulation of transcription from RNA polymerase II promoter]
GO:0006367 [transcription initiation from RNA polymerase II promoter]
GO:0010467 [gene expression]
Show all
 130 aa
14.8 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.