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Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & cytoplasmic bodies.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Antibody dilution
1:50
1:89
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028647
Antibody HPA030540
Standard validation
Approved
Figure description
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 82 cell types
Retrieval
HIER pH6
Antibody dilution
1:275
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028647
Antibody HPA030540
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414453)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.5, 26.4, 23.7, 21.9
26.5, 26.4, 23.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA028647
Antibody HPA030540
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
