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PGLYRP2

ANTIBODY INFORMATION
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA043568   HPA046311   HPA075237   sc-50470  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 12   12   16   7  

References

        

Proper citation

 Atlas Antibodies Cat#HPA043568, RRID:AB_2678559   Atlas Antibodies Cat#HPA046311, RRID:AB_2679621   Atlas Antibodies Cat#HPA075237, RRID:AB_2686739   Santa Cruz Biotechnology Cat#sc-50470, RRID:AB_1127187  

VALIDATION SUMMARY
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  
Immunocytochemistry

    Approved    
Immunohistochemistry

Approved   Approved     Approved  
Western blot

Supported   Supported     Supported  
Protein array

Supported   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Standard validation

     Approved    

       

Figure description

     Immunofluorescent staining of human cell line MCF7 shows localization to cell junctions.    

Antibody dilution

     1:63    

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Standard validation

 Approved   Approved     Approved  

       

Figure description

 Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.     Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 61 cell types		   RNA: detected in 3 tissues
Protein: detected in 71 cell types		     RNA: detected in 3 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:15   1:175     1:225  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		     Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 68, 62.2   68, 62.2, 19.5     68, 62.2, 19.5, 11.4  

Antibody dilution

 1:250   1:250     1:500  

PROTEIN ARRAY
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:12000   1:2500    

ANTIGEN INFORMATION
 
 

Antibody HPA043568

  

Antibody HPA046311

  

Antibody HPA075237

  

Antibody CAB033468

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 80   107   75    

Antigen sequence

 
VWGTLVLLQRLEPVHLQLQCMSQEQLAQVAANATKEFTEAFLGCPAIHPR
CRWGAAPYRGRPKLLQLPLGFLYVHHTYVP
  
LMSAPNSGPHNRLYHFLLGAWSLNATELDPCPLSPELLGLTKEVARHDVR
EGKEYGVVLAPDGSTVAVEPLLAGLEAGLQGRRVINLPLDSMAAPWETGD
TFPDVVA
  
DTLPSCAVRAGLLRPDYALLGHRQLVRTDCPGDALFDLLRTWPHFTATVK
PRPARSVSKRSRREPPPRTLPATDL
  

											 
																				

																		

										
											
Matching transcripts

												
																					PGLYRP2-001 - ENSP00000345968 [100%] 
PGLYRP2-002 - ENSP00000292609 [100%] 

											 
																						PGLYRP2-001 - ENSP00000345968 [100%] 
PGLYRP2-002 - ENSP00000292609 [100%] 
PGLYRP2-003 - ENSP00000472856 [86%] 

											 
																						PGLYRP2-001 - ENSP00000345968 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PGLYRP2-001
PGLYRP2-002
PGLYRP2-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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