CLPB - Antibodies - The Human Protein Atlas

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CLPB

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039005

HPA039006

HPA064571

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA039005, RRID:AB_2676305

Atlas Antibodies Cat#HPA039006, RRID:AB_10672767

Atlas Antibodies Cat#HPA064571, RRID:AB_2685290

VALIDATION SUMMARY

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 56 cell types

RNA: detected in 37 tissues
Protein: detected in 52 cell types

RNA: detected in 37 tissues
Protein: detected in 80 cell types

Retrieval

HIER pH6

Antibody dilution

1:175

1:300

1:1500

Literature conformity

No avaliable gene/protein characterization data.

Consistent with gene/protein characterization data.

RNA consistency

Not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

80.1, 78.7, 75.4, 74.5, 72.3, 57.7

Antibody dilution

1:250

1:640

PROTEIN ARRAY

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:6000

1:7700

ANTIGEN INFORMATION

Antibody HPA039005

Antibody HPA039006

Antibody HPA064571

Antigen

Recombinant protein fragment

Length (aa)

108

105

Antigen sequence

GKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQI
SDKITISKNFKENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLV
NKELNFWA

KRAKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRVVNQLAAAY
EQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSK
TRRLD

RRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDM
SEFQERHEVAKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLF

Matching transcripts

CLPB-001 - ENSP00000294053 [100%]
CLPB-002 - ENSP00000340385 [100%]
CLPB-003 - ENSP00000441518 [100%]
CLPB-004 - ENSP00000439746 [100%]
CLPB-006 - ENSP00000443822 [100%]
CLPB-201 - ENSP00000407296 [100%]

Other gene match

ANTIGEN VIEW

CLPB-001	CLPB-002	CLPB-003	CLPB-004	CLPB-006	CLPB-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.