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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PRKAA2
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PRKAA2

Gene description

Protein kinase, AMP-activated, alpha 2 catalytic subunit

Protein class

Enzymes
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Mixed
HPA (cell line):Cell line enhanced (RPTEC TERT1)
HPA (normal tissue):Tissue enhanced (heart muscle)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression mainly in heart, skeletal muscle and kidney.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data. Presumed off target binding observed and disregarded.

Reliability score

Approved based on 1 antibody.
HPA044540
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (favourable), endometrial cancer (unfavourable) and liver cancer (unfavourable).
Renal cancer p<0.001
Endometrial cancer p<0.001
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Mixed

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA044540

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Many renal cell carcinomas, breast cancers along with few melanomas, endometrial, occasional lung, pancreatic and liver cancer tissues showed moderate cytoplasmic positivity. Other cancer tissues were weakly stained or in general negative.

GENE INFORMATION

Gene name

PRKAA2 (HGNC Symbol)

Synonyms

AMPK, AMPKa2, PRKAA

Description

Protein kinase, AMP-activated, alpha 2 catalytic subunit (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]

Chromosome

1

Cytoband

p32.2

Chromosome location (bp)

56645322 - 56715335

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000162409 (version 83.38)

Entrez gene

5563

UniProt

P54646 (UniProt - Evidence at protein level)

neXtProt

NX_P54646

Antibodypedia

PRKAA2 antibodies

PROTEIN BROWSER

PRKAA2-001
PRKAA2-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PRKAA2-001
ENSP00000360290
ENST00000371244
P54646 [Direct mapping]
5'-AMP-activated protein kinase catalytic subunit alpha-2
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   CAMK Ser/Thr protein kinases
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT-SVM predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003682 [chromatin binding]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0004679 [AMP-activated protein kinase activity]
GO:0004712 [protein serine/threonine/tyrosine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006112 [energy reserve metabolic process]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006367 [transcription initiation from RNA polymerase II promoter]
GO:0006468 [protein phosphorylation]
GO:0006633 [fatty acid biosynthetic process]
GO:0006695 [cholesterol biosynthetic process]
GO:0006853 [carnitine shuttle]
GO:0006950 [response to stress]
GO:0006996 [organelle organization]
GO:0007005 [mitochondrion organization]
GO:0007050 [cell cycle arrest]
GO:0007165 [signal transduction]
GO:0008286 [insulin receptor signaling pathway]
GO:0008610 [lipid biosynthetic process]
GO:0010467 [gene expression]
GO:0010468 [regulation of gene expression]
GO:0010508 [positive regulation of autophagy]
GO:0014850 [response to muscle activity]
GO:0016055 [Wnt signaling pathway]
GO:0016236 [macroautophagy]
GO:0016239 [positive regulation of macroautophagy]
GO:0016241 [regulation of macroautophagy]
GO:0031588 [nucleotide-activated protein kinase complex]
GO:0031669 [cellular response to nutrient levels]
GO:0032007 [negative regulation of TOR signaling]
GO:0035174 [histone serine kinase activity]
GO:0035404 [histone-serine phosphorylation]
GO:0035556 [intracellular signal transduction]
GO:0035690 [cellular response to drug]
GO:0042149 [cellular response to glucose starvation]
GO:0042304 [regulation of fatty acid biosynthetic process]
GO:0042593 [glucose homeostasis]
GO:0042752 [regulation of circadian rhythm]
GO:0043066 [negative regulation of apoptotic process]
GO:0044255 [cellular lipid metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045821 [positive regulation of glycolytic process]
GO:0046872 [metal ion binding]
GO:0047322 [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity]
GO:0048511 [rhythmic process]
GO:0050405 [[acetyl-CoA carboxylase] kinase activity]
GO:0055089 [fatty acid homeostasis]
GO:0061024 [membrane organization]
GO:0071380 [cellular response to prostaglandin E stimulus]
GO:2000505 [regulation of energy homeostasis]
Show all
552 aa
62.3 kDa
No 1
PRKAA2-201
ENSP00000481378
ENST00000610361
A0A087WXX9 [Direct mapping]
5'-AMP-activated protein kinase catalytic subunit alpha-2
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT-SVM predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0006468 [protein phosphorylation]
GO:0031588 [nucleotide-activated protein kinase complex]
GO:0032007 [negative regulation of TOR signaling]
GO:0035174 [histone serine kinase activity]
GO:0035404 [histone-serine phosphorylation]
GO:0035556 [intracellular signal transduction]
GO:0042149 [cellular response to glucose starvation]
Show all
552 aa
62.3 kDa
No 1

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