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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:100
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA028555
Antibody HPA028564
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 26 tissues Protein: detected in 46 cell types
RNA: detected in 26 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028555
Antibody HPA028564
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407623)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407623)
Target mass (kDa)
30.8
30.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA028555
Antibody HPA028564
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
