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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:154
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA032058
Antibody HPA043197
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human esophagus shows moderate nuclear/nucleolar positivity in squamous epithelial cells.
Expression
RNA: detected in 8 tissues Protein: detected in 76 cell types
RNA: detected in 8 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:275
1:400
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA032058
Antibody HPA043197
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407615)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407615)
Target mass (kDa)
37.8, 30.7
37.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA032058
Antibody HPA043197
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
