Fields »
ABOUT
HELP
BLOG
KIF26B

ANTIBODY INFORMATION
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027709   HPA028478   HPA028561   HPA028562  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 6   6   6   6  

References

        

Proper citation

 Atlas Antibodies Cat#HPA027709, RRID:AB_10600215   Atlas Antibodies Cat#HPA028478, RRID:AB_10601231   Atlas Antibodies Cat#HPA028561, RRID:AB_10601960   Atlas Antibodies Cat#HPA028562, RRID:AB_10602417  

VALIDATION SUMMARY
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  
Immunocytochemistry

      Approved  
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & microtubules.  

Antibody dilution

       1:100  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells and cells in granular layer.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells and cells in granular layer.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 7 tissues
Protein: detected in 38 cell types		   RNA: detected in 7 tissues
Protein: detected in 58 cell types		   RNA: detected in 7 tissues
Protein: detected in 72 cell types		   RNA: detected in 7 tissues
Protein: detected in 39 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:65   1:75   1:50   1:450  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

       

Figure description

        

Target mass (kDa)

 223.9   223.9, 184.7   223.9, 184.7   223.9, 184.7  

Antibody dilution

 1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000   1:3000   1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA027709

  

Antibody HPA028478

  

Antibody HPA028561

  

Antibody HPA028562

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 59   90   93   86  

Antigen sequence

 
GIGTSSPGSLGGSPGFGTGSPGSGSGGGSSPGSDRGVWCENCNARLVELK
RQALRLLLP
  
TSFSGILQTSPPPAPPCLLRAVNKVKDTPGLGKVKVMLRICSTLARDTSE
SSSFLKVDPRKKQITLYDPLTCGGQNAFQKRGNQVPPKMF
  
GYIPMKTNITVYPCIAMSPRNIQEPEAPTATPKAGPTLAQSRESKENSAK
KEMKFEDPWLKREEEVKKETAHPNEEGMMRCETATGPSNAETR
  
SCHSFIAQTCFGHGEAMAEPVASEFVSSLQNTAVVCREKPKASPDNLLIL
SEMGDDSFNKAAPIKGCKISTVSKAMVTISNTANLS
 

Matching transcripts

 KIF26B-005 - ENSP00000385545 [100%]
  KIF26B-001 - ENSP00000355475 [100%]
KIF26B-005 - ENSP00000385545 [100%]
  KIF26B-001 - ENSP00000355475 [100%]
KIF26B-005 - ENSP00000385545 [100%]
  KIF26B-001 - ENSP00000355475 [100%]
KIF26B-005 - ENSP00000385545 [100%]
 

Other gene match

        
ANTIGEN VIEW
KIF26B-001
KIF26B-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.