We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.
Antibody dilution
1:49
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003910
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in subset of germinal center cells and non-germinal center cells.
Expression
RNA: detected in 32 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003910
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401671)
Target mass (kDa)
43.1, 33.8, 31
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA003910
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
